chemiluminescence imaging system  (Bio-Rad)

Journal: Materials Today Bio

Article Title: Multidimensional nano-ion composite hydrogel based on enzymatic blood glucose control, gas therapy and ion liquid permeation for repairing diabetic wounds

doi: 10.1016/j.mtbio.2026.103213

Figure Lengend Snippet: Gel-HMSN/GOx-LArg@IL promotes vascular neurogenesis and cell migration. A) Optical images of vascular formation by HUVECs subjected to different treatments. Scale bar: 200 μm. B) Cell migration experiments of HUVECs in different treatment groups. Scale bar: 200 μm. C) Immunofluorescence staining of β 3 -tubulin expression in BMSC cells of different treatment groups. Scale bar: 200 μm. D) Quantitative analysis of vascular length. E) Quantitative analysis of cell migration rate. F) Quantitative analysis of fluorescence intensity. Data are represented as mean ± SD (n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey's test ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001.

Article Snippet: Protein bands were visualized using a chemiluminescent substrate and imaged with a fluorescence & chemiluminescence imaging system (Servicebio).

Techniques: Migration, Immunofluorescence, Staining, Expressing, Fluorescence

Journal: Materials Today Bio

Article Title: Multidimensional nano-ion composite hydrogel based on enzymatic blood glucose control, gas therapy and ion liquid permeation for repairing diabetic wounds

doi: 10.1016/j.mtbio.2026.103213

Figure Lengend Snippet: Skin immunofluorescence staining and immunohistochemical analysis were performed in different treatment groups. A) Immunofluorescence staining images of NF200. β 3 -Tubulin. PGP9.5. CD31. VEGF and IL-6 in skin wounds on the 14th day after treatment. Scale = 400 μm. B) Immunohistochemical images of FGF2 on day 14 in wound tissue. Scale = 400 μm. C) Images of the immunohistochemistry of AGEs. Scale bar = 400 μm. D) Fluorescence intensity of NF200. E) Fluorescence intensity of β 3 Tubulin. F) Fluorescence intensity of PGP9.5. G) CD31-labeled micro vessel density. H) Fluorescence intensity of VEGF. I) Fluorescence intensity of IL-6. J) Integrated option density (IOD) of FGF2. K) IOD of AGEs. Data are represented as mean ± SD (n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey's test ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001.

Article Snippet: Protein bands were visualized using a chemiluminescent substrate and imaged with a fluorescence & chemiluminescence imaging system (Servicebio).

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Immunohistochemistry, Fluorescence, Labeling